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By combing the application and funding situation of general， young scholar and regional scholar programs from National Natural Science Foundation of China（NSFC） in field of integrated traditional Chinese and western medicine in 2023， this paper summarizes the distribution of supporting units， application and funding hotspots， and the problems of application and funding projects in this discipline， in order to provide a reference for applicants and supporting organizations to understand the hotspot dynamics and reporting requirements of the discipline. In 2023， the discipline of integrated traditional Chinese and western medicine received a total of 2 793 applications， and there were 1 254 applications for general programs， 1 278 applications for young scholar programs， and 261 applications for regional scholar programs. The amounts of project funding obtained by the three were 145， 164 and 35， respectively， and the funding rates were 11.56%， 12.83% and 13.41% in that order. From the situation of obtaining funding， the age distribution of the project leaders who obtained funding for the general， young scholar and regional scholar programs were mainly distributed in the age of 40-46， 30-34， 38-44 years， respectively. Within the supported programs， the Chinese medicine affiliations accounted for 55.52%. With respect to research subjects， the proportion of one single Chinese herbs， or monomers， or extracts accounted for 29.4%， but the proportion of Chinese herb pairs or prescriptions accounted for 47.1%. Research hotspots included ferroptosis， bile acid metabolism， macrophages， mitochondria， microglia， exosomes， intestinal flora， microecology and so on. The current research mainly focused on the common key problems of the advantageous diseases of Chinese and western integrative medicine， but still need to be improved in the basic theories of Chinese and western medicine and multidisciplinary cross-disciplinary research.
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OBJECTIVE To s tudy the improvement effects of sinapic acid on Aβ42-induced injury of PC 12 cells and the mechanism. METHODS PC12 cells were divided into five groups ：control group ，model group ，sinapic acid group ，phosphoinositide- 3-kinase（PI3K）inhibitor group and extracellular signal-regulated kinase （ERK）inhibitor group. Each inhibitor group was added with LY 294002 and U 0126（10 μmol/L）for 1 h；except for control group ，other four groups were treated with 2 μmol/L Aβ42 for 24 h to replicate the Alzheimer ’s disease cell model ；except for control group and model group ，other three groups were added with 100 μmol/L sinapic acid respectively. After 24 hours of continuous culture ，survival rate of PC 12 cells was detected and the morphology of PC 12 cells was observed. The content of Aβ42，mRNA expression of cAMP response element binding protein （CREB），protein expression of cyclic adenosine monophosphate （cAMP），protein kinase A （PKA），CREB signaling pathway and phosphorylated CREB （p-CREB）were detected. RESULTS After treated with sinapic acid ，the survival rate of PC 12 cells，mRNA expression of CREB and protein expressions of cAMP ，PKA and p-CREB were increased significantly （P＜0.05），while the content of Aβ42 was decreased significantly （P＜0.05）；cell morphology was significantly improved and synapses increased. After intervened with PI 3K and ERK inhibitors ，the survival rate of PC 12 cells，above mRNA and protein expressions were reversed significantly （P＜0.05 or P＜0.01）；cell morphology was irregular ，the fragments increased ，and the synaptic connections decreased. CONCLUSIONS Sinapic acid can improve the survival rate of PC 12 cells injured by A β 42，improve cell （No.2021-KYYWF-0349） morphology and decrease the content of Aβ42，the mechanism of which may be associated with promoting the gene transcription of CREB ， and activating cAMP/PKA/CREB signaling pathway.
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Lung cancer is the most common malignant tumor. As the first-generation oral small-molecule tyrosine kinase inhibitor independently developed in China, apatinib can block many kinds of signaling pathways with high selectivity, and play an anti-tumor effect by inhibiting tumor angiogenesis, and the adverse reactions are controllable. More and more studies have shown that apatinib can be used for the second-line and beyond treatment of patients with advanced non-small cell lung cancer. This article reviews the mechanisms and clinical studies of apatinib in treatment of lung cancer.
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Objective:To investigate the prevalence of cataract and its risk factors in the Jiu Shi Hospital of Xinjiang Production and Construction Corps and Han and Kazak people aged 50 years old and over in Emin County, Tacheng area, Xinjiang.Methods:A cross-sectional study was conducted.An epidemiological survey on the prevalence of cataract among people aged 50 years old and over was performed by the Department of Ophthalmology of North China University of Science and Technology Affiliated Hospital in the Jiu Shi Hospital of Xinjiang Production and Construction Corps and Emin County from October 2015 to June 2018.The basic sampling units (BSUs) were divided according to the administrative regions, with about 1 000 people in each administrative region.With a population exceeding 1 500, sample units would be divided into more than two BSUs according to the population.Xinjiang Tacheng area was divided into 240 BSUs, and 12 BSUs were selected by random number table method for inspection.With the lens opacity classification system Ⅱ (LOCS Ⅱ) as the diagnostic standard, the prevalence rate of cataract in different nationalities and different ages in this area was evaluated, and the relationship between age, nationality, sex, hypertension, diabetes, smoking, hyperlipidemia, coronary heart disease, ultraviolet irradiation time and the prevalence rate of cataract was analyzed.This study protocol was approved by the Ethics Committee of the Jiu Shi Hospital of Xinjiang Production and Construction Corps (No.SW-201501-04).Results:The prevalence rate of cataract among people aged 50 and over in the Jiu Shi Hospital of Xinjiang Production and Construction Corps and Emin County was 76.34%.The prevalence rate of cataract in 50-<60-year-old group, 60-<70-year-old group, 70-<80-year-old group and ≥80-year-old group was 23.30%, 77.40%, 89.60% and 100.00%, respectively.There were significant differences in the prevalence among people between different ages, different nationalities, with and without hypertension, with and without diabetes, with and without hyperlipidemia (all at P<0.01). There was no significant difference in the prevalence among people between different sexes, with and without coronary heart disease, smoking or not, different ultraviolet irradiation time (all at P>0.05). Multiple logistic regression analysis showed that age (50-<60 years old: [ OR=39.93, 95% CI: 20.77-76.77; 60-<70 years old: OR=3.81, 95% CI: 2.05-7.08; 70-<80 years old: OR=1.72, 95% CI: 0.92-3.22]), hypertension ( OR=1.54, 95% CI: 1.13-2.11) and hyperlipidemia ( OR=1.41, 95% CI: 1.10-2.03) were independent risk factors for cataract. Conclusions:The incidence of cataract in the Jiu Shi Hospital of Xinjiang Production and Construction Corps and people aged 50 and over in Emin County is higher than that in other parts of China, in which age, hypertension and hyperlipidemia are independent risk factors for cataract.
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Objective:To systematically evaluate the efficacy of benzathine penicillin combined with ceftriaxone on the negative conversion rate of the rapid plasma reagin circle card test/toluidine red unheated serum test (RPR/TRUST) in the treatment of early syphilis.Methods:According to the search strategy, online databases (PubMed, Web of science, Embase, Cochrane Library, CNKI, Wanfang and VIP) were searched for case-control studies on benzathine penicillin combined with ceftriaxone in the treatment of early syphilis. The Newcastle Ottawa scale (NOS) was used to evaluate the quality of the included articles, and the RevMan5.3 software to analyze the negative conversion rate of RPR/TRUST.Results:A total of 14 case-control studies were included, including 1 160 syphilis patients (585 in the combination treatment group and 575 in the benzathine penicillin alone group) . According to the meta-analysis, the negative conversion rate of serum RPR/TRUST was significantly higher in the combination treatment group than in the benzathine penicillin alone group ( OR=3.70, 95% CI[2.71, 5.06], P<0.001) . Subgroup analysis based on the follow-up duration, the negative conversion rate of serum RPR/TRUST was also significantly higher in the combination treatment group than in the benzathine penicillin alone group after 3-month ( OR=3.68, 95% CI [2.26, 5.98], P<0.001) , 6-month ( OR=3.11, 95% CI[2.26, 4.27], P<0.001) and 12-month treatment ( OR=4.35, 95% CI[2.81, 6.73], P<0.001) . Conclusion:Compared with benzathine penicillin, benzathine penicillin combined with ceftriaxone can more effectively promote serum RPR/TRUST to turn negative in the treatment of early syphilis.
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OBJECTIVE：To study the improvement effects of sinapic acid （SA）on PC 12 cell damage induced by Aβ1-42，and to investigate its effect on brain-derived neurotrophic factor （BDNF）/tyrosine kinase B （TrkB）/extracellular signal-regulated kinase （ERK）signaling pathway. METHODS ：PC12 cells were divided into blank group ，model group ，SA low-dose and high-dose groups（50，100 μmol/L）. Except for blank group ，cell damage was induced by Aβ1-42 in other groups ；24 h after modeling ， administration groups were added with the corresponding solution and cultured for 24 h. Morphological changes of cells in each group were observed. Cell survival rate ，mRNA expression and protein level of BDNF ，protein expression of TrkB ，ERK1/2 and phosphorylated ERK 1/2（p-ERK1/2）were detected. p-ERK/ERK ratio was calculated. RESULTS ：Compared with blank group ，the model group had shorter synapses ，looser intercellular junctions ，poor adhesion ，dim cytoplasm and more granules in cytoplasm. Cell survival rate and mRNA expression and protein level of BDNF ，the relative expression of TrkB and p-ERK 1/2 protein，p-ERK/ ERK ratio were significantly decreased （P＜0.05 or P＜0.01）. Compared with model group ，in SA high-dose group the pathological changes of the cells were significantly improved ，the survival rate of the cells ，the mRNA expression and protein level of BDNF，the relative expression of TrkB and p-ERK 1/2 protein， p-ERK/ERK ratio were significantly increased （P＜0.05 or P＜ 0.01）. CONCLUSIONS：SA can i mprove PC 12 cells damage induced by Aβ1-42，the mechanism of which may be associated with activating BDNF/TrkB/ERK signaling pathway. qq.com
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OBJECTIVE：To investigate the mechanism of sinapic acid （SA）against PC 12 cell injury induced by Amyloid β1-42 protein（Aβ1-42）based on PI 3K/Akt/GSK3β signaling pathway. METHODS：PC12 cells of rats were randomly divided into control group，Aβ group（Aβ1-42 2 μmol/L），Aβ+SA group（Aβ1-42 2 μmol/L+SA100 μmol/L），Aβ+SA+LY group [Aβ1-42 2 μmol/L+SA 100 μmol/L+LY294002（PI3K inhibitor ）10 μmol/L]，Aβ+LY group（Aβ1-42 2 μmol/L+LY294002 10 μmol/L）and LY group （LY294002 10 μmol/L）. Except for control group and LY group ，the cells of other groups were replicated the damage model with Aβ1-42. After 24 hours of culture ，the morphology of cells was obsened in each group with a microscope ，and MTT assay was adopted to determine the cell viability of PC 12 cells in each group. Western blotting assay was used to detect the expression of PI 3K，p-PI3K， Akt，p-Akt，GSK3β and p-GSK3β in cells of each group. RESULTS：Compared with control group ，the number of cells decreased and some synaptic breaks disappeared in Aβ group while cell viability，ratio of p-PI 3K/PI3K，p-Akt/Akt and p-GSK 3β/GSK3β in Aβ group were decreased significantly（P＜0.05 or P＜0.01）. Compared with Aβ group，the cells became round and synapses became more in Aβ+SA group while cell viability，the ratio of p-PI 3K/PI3K，p-Akt/Akt and p-GSK 3β/GSK3β were increased significantly（P＜0.05）. Compared with Aβ+SA group，some synaptic breaks occurred in Aβ+SA+LY group while cell viability， the ratio of p-PI 3K/PI3K，p-Akt/Akt and p-GSK 3β/GSK3β were decreased significantly（P＜0.05）；Aβ+LY group had more cell debris，and t he cell viability was decreased ，but the difference was not significant ，and the ratio of p-PI 3K/PI3K，p-Akt/Akt and p-GSK3 β/GSK3 β had no significant change （P＞0.05）; LY294002 alone had no significant effect on morphology ，cellviability and the ratio of p-PI 3K/PI3K，p-Akt/Akt or p-GSK 3β/ GSK3 β （P＞0.05）. CONCLUSIONS ： SA may play aprotective role against PC 12 cell injury induced by A β 1-42 through activating PI 3K/Akt/GSK-3β.
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Objective To summarize the care points of nursing to patients with maxillary defect repaired by 3D printing individualized titanium mesh. Methods Nursing staff cooperated with doctors to control the disease of the whole body and prevent oral infection by performing psychological nursing, keeping the respiratory tract smooth after operation, preventing bleeding and infection of the wound, conducting oral, eye and pain care, preventing cerebrospinal fluid leakage, intracranial infection and venous blood thrombus of lower extremity and helping the patients with rehabilitation training. Results The hospitalization time of 5 patients was 11～20d with an average of (14.80 ± 3.31) d. 1 case had cerebrospinal fluid leakage during the operation and 1 had postoperative corneal edema. After treatment and nursing, all the patients were discharged from the hospital. Follow-up of 6 months showed no recurrence and good appearance in the maxillofacial region. Conclusion The nursing measures for the patients with to patients with maxillary defect repaired by 3D printing individualized titanium mesh include respiratory tract management, oral cavity, eye and pain nursing, prevention of bleeding and infection of wound, prevention of cerebrospinal fluid leakage, intracranial infection and venous thrombosis of lower extremities, which can promote the recovery of the patients soon.
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OBJECTIVE:To provide reference for improving the utilization ratio of emergency contraceptive(EC)pills and re-ducing unwanted pregnancy. METHODS:Among 458 women with unwanted pregnancy in our hospital,self-designed question-naires were used to survey the respondents'knowledge and utilization of EC pills,reasons for not using,sources of EC knowledge and drug availability. The results were analyzed statistically. RESULTS:Totally 458 questionnaires were distributed and then 426 valid questionnaires were collected with effective rate of 93.0%. Among respondents,the awareness rate of EC was 81.2%(346/426),and the rate of those receiving higher education was higher than that of other education levels(P<0.001). 56.3%(240/426) of the respondents had ever used EC pills at one time,the utilization ratio of EC pills were higher in women less than 30-year-old and receiving higher education than other age and educational levels(P<0.01). Only 20.2%(86/426)of the respondents ever used EC pills after the last menstrual period and only 3.5%(3/86) of them took EC pills correctly. Not understanding or knowing EC was the main reason for not using EC pills,accounting for 60.6%(206/340). The highest proportion of source which the respon-dents got EC knowledge was network,accounting for 53.5%(185/346). 37.0%(81/219) of the respondents who had ever pur-chased drugs couldn't purchase EC pills conveniently. CONCLUSIONS:The utilization ratio of EC pills in women with unwanted pregnancy needs to be improved and non-standard drug use is more common. It indicates that health education of contraception still need to be strengthened and the availability of EC pills improved.
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Objective To investigate the status of high-risk human papilloma virus(HR-HPV)infection and natural clearance in males whose wife have been infected,and to analysis the coincidence rates of HPV subtypes in couples.Methods Urethra epithelial cells and urethral secretions of 246 males whose wife infected with HR-HPV have been enrolled in the study to detect 13 kinds of HPV subtypes and redetect after 1 year.Results The natural clearance rate of HR-HPV in male was 31.82％(35/110).The infection rate was 44.72％(110/246)in initial detection,while 30.49％(75/246) in redetection,the difference was statistically significant(χ2=10.612,P<0.05).The coincidence rate of HPV subtypes in couples was 15.04％(37/246)in initial detection,while 8.94％(22/246)in redetection,the difference was statistically significant(χ2=4.333,P<0.05).The frequency percentage of HPV16,18 increased significantly in male,the difference was statistically significant(χ2=35.991,P<0.05).Conclusion HR-HPV can be cross-transmitted between infected women and their spouses.It is necessary to detect for her husband once women have been detected high-risk HPV positive.
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BACKGROUND: Anti-virus therapy is not suitable for the patients who have asymptomatic human immunodeficiency virus (HIV); otherwise, it is significant for early intervention of Chinese herbs to cure asymptomatic HIV.OBJECTIVE: To observe the interventional effects of fuzheng paidu pian on level of CD4+T lymphocytes and changes of plasma viral vector expression in early phase of asymptomatic HIV infection.DESIGN : Observational contrast study.SETTING: Institute of Acquired Immunodeficiency Syndrome (AIDS), Henan College of Traditional Chinese Medicine.PARTICIPANTS: A total of 69 asymptomatic HIV patients, who were infected by paid blood, were randomly selected from some countryside in November 2005. There were 39 males and 30 females. Their ages ranged from 30 to 61 years and the mean age was (43±8) years, and the mean course was (13±3) years. Inclusion criteria: ① coincident with diagnostic criteria of asymptomatic HIV infection published by National AIDS committee (modified in 2005); ②from 18 to 65 years old; ③ 200/mm3 ≤CD4+T lymphocyte count ＜ 600/mm3; ④ Patients did not treat with antiviral and other drugs; ⑤ All patients provided the confirmed consent. Exclusion criteria: ① severe liver and renal malfunction,combining with cardio-cerebral vascular disease and severe primary diseases of lung and hematopoietic system,mental diseases; ② primary immunodeficiency, secondary immunodeficiency induced by hormone and radiation therapy, hematologic disease; ③ pregnant or lactational women. Another 25 asymptomatic HIV patients were randomly selected.and regarded as the blank group. Inclusion and exclusion criteria were as the same as those in the treatment group.METHODS: Patients in the treatment group were treated with fuzheng paidu pian (consisting of huangqi, raw baizhu,fangfeng, baihuashe shecao, ganca o, etc.; Aolinte Pharmaceutical Factory of Henan Institute of Traditional Chinese Medicine; batch number: 050601) by using warm boiled water, 5 tablets once (eqUal to 6.6 g raw materials) for three times a day. Three months were regarded as a course and the treatment was performed four courses in total. Patients in the blank group were not treated with any drugs.MAIN OUTCOME MEASURES: ① Values of CD4+, CD8+ and CD4+/CD8+ in the treatment group before and after treatment;CD4+T lymphocyte count in the blank group after 6 months; ② Viral vector was detected by using reverse transcription polymerase chain reaction (RT-PCT) in the treatment group.RESULTS: All 69 patients in the treatment group and 25 patients in the blank group were involved in the final analysis. ①CD4+ value was decreased in the blank group after 6 months, and there was significant difference before and after 6 months based on α =0.05 level (P ＜ 0.05).However, CD4+ value was increased in the treatment group. ② Viral vector was randomly detected from 23 patients in the treatment group. As compared with before treatment, plasma viral vector was decreased in 9 patients at 6 months after treatment and in 12 patients at 1 year after treatment. The effective rate was 91.30%.CONCLUSION: Fuzheng paidu pian can improve immunological function of asymptomatic HIV patients and decrease viral vector in some cases during early interventional period.
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Objective:To explore the etiopathogenisis and pathogenesis of AIDS,provide the theoretical basis for TCM treatment based on syndrome differentiation.Methods:15 TCM experts who engaged in prevention and control of AIDS were investigated by the questionnaires,and the results were analysed.Results:In the experts' opinion,epidemic toxicity,damp-toxin,dampness pathogen and heat pathogen are the common etiopathogenisis of AIDS.Deficiency of primordial qi,yang deficiency of spleen and kidney and deficiency of both qi and yin are the common pathogenesis.The common invasion sits of pathogenic factors are in sequence of the spleen,kidney,lung,stomach and liver.Conclusion: AIDS is caused by damp-heat and epidemic toxicity,human immunodeficiency virus(HIV) has been confirmed to be the direct pathogenesis.The pathological characteristic is HIV toxicity danmaging the primordial qi.Pathological changes implicate mainly the spleen and the kidney.
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Objective To study the genetic toxicity of 3 brands of detergents to the root tip cells of Vicia faba in the inducing time and the inducing way(continual mutagenesis,accumulative mutagenesis) with micronucleus and the chromosomal aberration Methods The root tip cells of Vicia faba were taken as the subjects,the genetic safety tests were conducted on those materials treated with detergent of a certain concentration(0.25%) for 2、4、6 h of continual mutagenesis and accumulative mutagenesis respectively.Distilled water was used as the control.Results The continual mutagenesis showed that all the three detergents could induce the root tip cells of Vicia faba to produce micronucleus and chromosomal aberration and as the mutagenic time prolonged,at 6 h,the changes became weak.The genetic toxicity in the accumulative mutagenesis group was greater than that in the continual mutagenesis group.Conclusion According to the results of the present paper,to some extent,detergents may produce genetic toxicity.
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AIM: To study the effect of effective parts of Acanthopanax senticosus on Parkinson's disease mouse model(from C_(57) BL/6 strain) concerning Gammaamino butyric acid(GABA),nigra-striatum tyrosine hydrozylase(TH) and hippocampal glial fibrillary acidic protein(GFAP). METHODS: In MPTP(1-methyl-4-phenyl-1,2,3,6-tetrahydropytidine)-induced PD mouse model(accumulative dose amounted to 150 mg/kg) pole-climbing time was determined of mice after receiving effective parts of Acanthopanax senticosus for 20 d,and GARA,TH and GFAP expression were assayed simultaneously with the help of immunohistochemical method. RESULTS: Mice's pole-climbing time was measured,GABA and TH made a significant difference between treated group and control group(P